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. 2018 May 9;8:7367. doi: 10.1038/s41598-018-25668-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RBM3 regulates myotube formation in vitro. Satellite cells maintained under differentiating conditions were transfected with RBM3 siRNA (si), an EYFP-RBM3 expression construct (o/x), or subjected to mock transfection (con). (a–c) Images of myoblasts in the indicated RBM3 expression conditions. siRNA-mediated knockdown of RBM3 (b) impaired myotube formation from differentiating cells, whereas RBM3 overexpression (c) enhanced it. (d–g) Graphs summarizing the length (d) and area (e) of formed myotubes, the percentage of all cells incorporated into myotubes containing at least 2 nuclei (mean ± SEM; n = 43, 14, 17 for con, si, o/x, respectively; *p < 0.05, 1-tailed t-test) (f) and the mean nuclei count (g) of all cells in each RBM3 expression condition (n = 135, 127, 43 for con, si, o/x, respectively; *p < 0.05, 1-tailed t-test).