Fig. 2.

RNAi screen reveals BAR domain proteins involved in CG endocytosis. a List of Drosophila proteins in the PFAM database that contained one of the following BAR domains, PFAM IDs—PF06456, PF09325, PF06456, PF00611 and PF08397. The list was filtered to remove duplicates to give 18 genes. b The histogram shows normalised 5-min fluid-phase uptake in S2R⁺ cells treated with 10 µg of dsRNA for 4 days as indicated with dsRNA against GBF1 (garz) as positive, and GFP as negative controls. In a single experiment, mean uptake of one of GFP dsRNA coverslip was used to normalise the mean for rest of the coverslips. Data were pooled from three independent experiments and the cell numbers are indicated in the graph. The bars in green are significantly different from GFP dsRNA using two-sample t-test (p value <0.05). Version 27 of the PFAM database was used to generate the list