Table 1.

pH pulsing assay data set

Molecule	% Coloc	# Spots SecGPI	# Spots Random	# Cell and Experiment
CDC42	56	219	3428	17 and 6
ARF1	62	411	4952	12 and 3
GBF1	62	132	1917	6 and 2
IRSp53	60	309	4439	7 and 3
ARP3	48	170	3277	16 and 6
Lifeact	61	244	4277	7 and 3
PICK1	77	121	1968	22 and 10
N-WASP	27	256	1827	9 and 3
Clathrin	30	437	1801	8 and 4
Dynamin	34	130	1866	10 and 4

See section titled pH pulsing analysis (Methods and S.I) for details