Skip to main content
. 2018 Apr 2;7(5):1988–2002. doi: 10.1002/cam4.1455

Figure 4.

Figure 4

Direct regulation of NCAPG by miR‐99a‐3p in PCa cells. (A) NCAPG mRNA expression was evaluated using qRT‐PCR in PC3, DU145, and C4‐2 cells 48 h after transfection with miR‐99a‐3p. GAPDH was used as an internal control. *P < 0.0001. (B) NCAPG protein expression was evaluated by Western blotting in PC3, DU145, and C4‐2 cells 72 h after transfection with miR‐99a‐3p. (C) miR‐99a‐3p binding sites in the 3′‐UTR of NCAPG mRNA. Dual‐luciferase reporter assays in PC3 using vectors encoding a putative miR‐99a‐3p target site in the NCAPG 3′‐UTR (positions 462–468). Data were normalized by expression ratios of Renilla/firefly luciferase activities. *P < 0.0001.