Figure 1.

Model outlining regulatory pathways affecting progression through molt. During intermolt, production of eyestalk MIH neuropeptide inhibits mTOR signaling and YO ecdysteroid synthesis. Eyestalk ablation triggers entry into premolt and ecdysteroid production via mTOR signaling activation. mTOR signaling invokes TGFβ signaling, leading to progression into mid- premolt, YO commitment through mid and late premolt, sustained ecdysteroidogenesis and concomitant ecdysis. mTOR inhibition via rapamycin injection depresses ecdysteroid synthesis and feeds back on mTOR and TGFβ signaling pathways.