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. 2018 May 9;8:7307. doi: 10.1038/s41598-018-25368-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of genes assigned to the insect hormone biosynthetic (IHB) pathway in the G. lateralis YO/I/DRESA transcriptome. KAAS was used to identify genes annotated by BLAST against the specific insect database (Section 4.5) and assigned to a KEGG pathway^38–40. (A) IHB pathway components/genes. Colored boxes indicate the presence of the genes identified in the ESA database. A pink colored box signifies the presence of an annotated gene that does not show a change in expression levels; a green box indicates a significant increase in premolt transcript abundance relative to intermolt while a yellow box indicates a decrease in transcript abundance. (B) Heat map showing the log2-fold change of the FPKM values of the DEGs.