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. 2018 May 9;8:7321. doi: 10.1038/s41598-018-25626-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of GFP-positive cells in lumbar DRGs and spinal cord following intraplantar administration of AAV2/9-CBA-Cre-GFP at postnatal day 5. (A) Quantification of GFP positive cells in lumbar dorsal root ganglia (L3-5) following the intraplantar delivery of AAV2/9-CBA-Cre-GFP. Data are the mean +/− S.E.M. of 3 mice for which the % of GFP-labeled neurons observed in 8 to 18 sections per mice has been averaged. (B) Representative photomicrographs illustrating GFP distribution in lumbar and trigeminal ganglia. (C) Representative photomicrographs illustrating GFP (absence of) and tdTomato staining in the lumbar spinal cord of an animal co-injected with AAV2/9-CBA-Cre-GFP and emCBA-Flex-tdTomato-WPRE are shown. Scale bars = 100 µm.