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. 2018 May 9;8:7321. doi: 10.1038/s41598-018-25626-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of GFP-positive cells in lumbar dorsal root ganglia and spinal cord following the intrathecal administration of AAV2/9-CBA-Cre-GFP. Representative photomicrographs illustrating GFP distribution in lumbar dorsal root ganglia (A) and the lumbar spinal cord (B, the dorsal horn is shown) of a mouse injected intrathecally with the AAV2/9-CBA-Cre-GFP virus at the adult age (25 days old; n = 4 mice). A representative photomicrograph illustrating the expression of tdTomato in the lumbar spinal cord of an animal co-injected with AAV2/9-CBA-Cre-GFP and AAV-emCBA-Flex-tdTomato-WPRE is also shown (C). (D–F) Nuclear GFP staining and tdTomato expression in a lumbar DRG of an animal co-injected with AAV2/9-CBA-Cre-GFP and AAV-emCBA-Flex-tdTomato-WPRE are shown. For panels C–F, images are representative of what has been observed in 3 mice. Scale bars = 100 µm.