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. 2018 May 3;9:542. doi: 10.3389/fpls.2018.00542

FIGURE 2.

FIGURE 2

Response of OsALMT4 expression to abiotic stresses. OsALMT4 expression in the shoot (A) and root (B) tissue of wild-type rice seedlings was measured using qRT-PCR after 6 and 24 h exposure to various treatments added to the nutrient solution including 200 mM NaCl, 350 mM mannitol (same osmolarity as the NaCl treatment), a low-Cl- solution, and 300 μM H2O2. Other treatments were low pH (pH 4.5) with or without 100 μM AlCl3, and either continual light (∼500 μmol m-2s-1) or continual darkness. The dashed line shows qRT-PCR data collected at the same time from control plants grown in parallel in standard hydroponic treatments. Expression measurements used two reference genes (GAPDH and eEF-1α) combined to provide a single result according to the software in Bio-Rad CFX ManagerTM. Data show mean and SE from three biological replicates where each replicate is the mean of three technical replicates. An asterisk indicates a significant difference (P < 0.05) between the treatment and control at the same time using a t-test.