Figure 1.

CpG-induced B10⁺ cells are more prone to produce pro-inflammatory cytokines than B10^neg cells. PBMCs were isolated from whole blood of healthy donors, stimulated 24 h with CpG, and stained intracellularly for tumor necrosis factor α (TNFα) (A) IFNγ (B), and IL-10. Cytokine production was assessed by flow cytometry in B10⁺ subset and B10^neg subset. In (C,D), TNFα and IFNγ concentration in the supernatants after 24 h of culture of isolated B10⁺ and B10^neg were measured by ELISA. Results are shown as median ± interquartile ranges and Wilcoxon matched pairs test was used for statistical analysis. n = 7–11.