Figure 2.

CpG induced B10⁺ cells from healthy subjects increased regulatory T (Treg) cells and Tr1 differentiation from naïve T cells. Sorted B10⁺ cells or B10^neg cells were co-cultured for 72 h with autologous CD4⁺CD25⁻cells or CD4⁺CD45RA⁺CD62L⁺ naïve T cells stimulated with anti CD3. Treg cells, defined as CD4⁺CD25^hiCD127^lo/neg, and Tr1, defined by CD4⁺IL-10⁺, were assessed by flow cytometry. Representative flow cytometry plot (gated on CD4⁺ cells) and percentages of CD4⁺CD25⁻ cells conversion by B10⁺ or B10^neg into Treg cells (A,B) or Tr1 (C,D) cells are presented. (E,F) Flow cytometry plot and graph presenting the conversion of CD4⁺CD45RA⁺CD62L⁺ by B10⁺ cells or B10^neg cells. Each connected pair of dots represents individual donor and results were obtained from independent experiments done in seven patients. p Values were calculated with Wilcoxon matched pairs test.