Figure 6.

B10⁺ cells from rheumatoid arthritis (RA) patients expressed less PD-L1 and more PD-L2 than B10⁺cells of healthy controls (HC). PBMCs were cultured for 24 h with CpG. Percentages of PD-L1 (A) and PD-L2 (B) positive cells among B10⁺ and B10^neg cells subsets were assessed in HC and in RA patients by flow cytometry. Representative flow cytometry plots and ratios of B10⁺ PD-L2⁺/B10⁺PD-L1⁺ cells are shown in (C). (D) Representative overlays and median of fluorescence intensity (MFI) showing PD-L2 and PD-L1 expression levels on B10⁺ cells. Medians and interquartile ranges are represented in the graphs. p values were calculated with Wilcoxon matched test for paired data or Mann–Whitney test for comparison between RA and HC.