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. 2018 May 3;9:480. doi: 10.3389/fphys.2018.00480

FIGURE 4.

FIGURE 4

Analysis of superoxide in renal rat ILA. (A) Representative images of vascular rings loaded with DHE under control conditions and after H/R treatment (DHE concentration: 2∗10-5 mol/l, magnification: 200×; scale bar = 400 μm) (B) Analysis of DHE fluorescence showed no significant differences between both groups. (C) H/R and simultaneous inhibition of superoxide, using the superoxide dismutase mimetic tempol, did not reverse the H/R effect on endothelium-independent relaxation in isolated rat renal ILA precontracted with PE. [EC50: 3.34∗10-8 mol/l (control) vs. 6.75∗10-8 mol/l (H/R); P < 0.05]. (D) Relative x-fold mRNA expression of NADPH oxidase enzymes (NOX) normalized to 18SrRNA after H/R relative to control conditions. The analyzed NOX were not differentially regulated following H/R. The average mRNA expression level under control conditions was arbitrarily given a value of 1 (20) (∗P < 0.05; compared to control; Mann–Whitney Test; n = 8). Mean ± SEM. (∗) indicates significant differences.