Table 1.

Nt sequences of fluorescently modified oligonucleotides used for the RNase assays and affinity determination assays.

Name	Sequence	Length
RNA oligonucleotides
mIL-682–106 3′FAM	5′-UGUUGUUCUCUACGAAGAACUGACA-3′-FAM	25 nt
mIL-682–106 5′FAM	FAM-5′-UGUUGUUCUCUACGAAGAACUGACA-3′	25 nt
mIL-682–106 RS	FAM-5′-ACAGUCAAGACUACGAUCUUGUUGU−3′	25 nt
mIL-682–106 YR	FAM-5′-UGUUGUACACUACGAUGUACUGACA-3′	25 nt
mIL-685–101 short stem	FAM-5′-UGUUCUCUACGAAGAAC-3′	17 nt
hIL-682–99	FAM-5′-UGUUCUCUAUGGAGAACU-3′	18 nt
consensus stem-loop	FAM-5′-UGGAAAGUAUCUUUCCU-3′	17 nt
mIL-682–93	FAM-5′-UGUUGUUCUCUA-3'	12 nt
mIL-682–88	FAM-5′-UGUUGUU-3'	7 nt
poly-U	FAM-5′-UUUUUUUUUUUU-3'	12 nt
mIL-682–106 int. ACA	FAM-5′-UGUACAUCUCUACGAAGAUGUUACA-3′	25 nt
mIL-683–98 ter. ACA	FAM-5′-ACAGUUCUCUAUGGAGAACUGU−3′	22 nt
mIL-685–93 ter. ACA	FAM-5′-ACAUGUUCUCUA-3′	12 nt
mIL-61–45	FAM-5′-UGCGUUAUGCCUAAGCAUAUCAGUUU GUGGACAUUCCUCACUGUG-3′	45 nt
DNA oligonucleotides
mIL-682–93 ssDNA	FAM-5′-TGTTGTTCTCTA-3′	12 nt
mIL-682–93 dsDNA	FAM-5′-TGTTGTTCTCTA-3′ 3′-ACAACAAGAGAT-5′	12 bp

Nts that form loop fragments of stem-loop structures are underlined. Sequences with numbered residues are part of the 3′UTR of transcripts from mouse or human IL-6. These sequences were numbered such that the first nt after the stop codon of the coding sequence is marked as 0. RS – reverse stem modification of mIL-6_82–106 (altered nts are in bold). YR – purine and pyrimidine residue modification of mIL-6_82–106 (altered nts are in bold)_.