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. 2018 May 9;8:7332. doi: 10.1038/s41598-018-25351-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Algorithm for the data analysis used in the assessment of the ICG washout ratio. T_M - time of maximum amplitude of the signal, T_B – time at which the baseline signal level was obtained (20 seconds after T_M). Averaged signal amplitude $\bar{S_{B}}$ was calculated within the ΔT_B period. T_E – time at which the late signal level was obtained (180 seconds after T_B). $\bar{S_{E}}$ - averaged signal amplitude within the ΔT_E period. (b) Examples of normalized time courses of variance of the statistical moments of the distributions of times of arrival (DTAs) of fluorescence photons (ΔVF) acquired during injection of ICG in a control subject, patient with brain edema and patient with brain death. R_VF variables calculated from these signals (ΔVF) were most significant in study groups differentiation (please, see Results section). For presentation purposes signals obtained in different subjects/patients were aligned by their maximum value.