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. 2018 May 3;9:581. doi: 10.3389/fpls.2018.00581

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Copyright © 2018 Rydahl, Hansen, Kračun and Mravec.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Generation and examples of mAb applications. (A) Simplified scheme of the standard procedure for anti-glycan mAb production. Unlike whole proteins, glycans are conjugated to a carrier molecule (typically BSA or KLH) before immunization. (B) Generated mAbs can be used in vitro to profile cell wall extracts in microarray applications or in ELISA-based methods like epitope detection chromatography providing also more structural information. (C) MAbs can be also used in situ for localization of the target molecules in situ on various types of plant material whole mount or sectioned by various methods.