Table 1.
A selection of commonly used antibodies related to pectins.
| Ab | Epitope | Source | References | |
|---|---|---|---|---|
| Homogalacturonan | JIM5 | HG with a low DE (range of partially methyl-esterified/unesterified HG) | Rat | Knox et al., 1990; Willats et al., 2000; Clausen et al., 2003; Verhertbruggen et al., 2009a |
| JIM7 | HG with a high DE | Rat | Knox et al., 1990; Willats et al., 2000; Clausen et al., 2003; Verhertbruggen et al., 2009a | |
| LM18 | HG, partially methylesterified or unesterified | Rat | Verhertbruggen et al., 2009a | |
| LM19 | HG, preferably unesterified (more selective than JIM5) | Rat | Verhertbruggen et al., 2009a | |
| LM20 | HG with a high DE (more selective than JIM7) | Rat | Verhertbruggen et al., 2009a | |
| CCRC-M130/M34 | HG with a high DE (cluster with JIM7) | Mouse | Pattathil et al., 2010 | |
| CCRC-M38 | Unesterified HG; DP>5 | Mouse | Pattathil et al., 2010 | |
| 2F4 | Calcium crosslinked HG (egg boxes) | Mouse | Liners et al., 1989 | |
| LM8 | Xylogalacturonan | Rat | Willats et al., 2004 | |
| LM7 | Partially methylesterified HG (non-blockwise de-esterification processes) also alginates | Rat | Clausen et al., 2003; Torode et al., 2015 | |
| PAM1 | Long stretches (over 30 units) of unesterified HG | phage display (scFv) | Willats et al., 1999, 2000; Manfield et al., 2005 | |
| RG-I | CCRC-M60 | Rhamnogalacturonan I and AGP | Mouse | Pattathil et al., 2010 |
| INRA-RU1/RU2 | Backbone of rhamnogalacturonan I | Mouse | Ralet et al., 2010; Ruprecht et al., 2017 | |
| CCRC-M14/M35/M36/M69/M129 | Backbone of rhamnogalacturonan I | Mouse | Ruprecht et al., 2017 | |
| CCRC-M2 | Rhamnogalacturonan Ia | Mouse | Pattathil et al., 2010 | |
| LM5 | (1 → 4)-β-D-galactan with at least three galactose units at non-reducing end. | Rat | Jones et al., 1997; Andersen et al., 2016; Torode et al., 2017 | |
| XD3 | (1 → 4)-β-D-galactan | Phage (scFv) | Shinohara et al., 2015 | |
| CCRC-M7 | 6-linked β-D-Gal oligomers that contain arabinose | Mouse | Pattathil et al., 2010 | |
| LM6 | (1 → 5)-α-L-arabinan / AGP epitopes | Rat | Willats et al., 1998; Verhertbruggen et al., 2009b | |
| LM13 | Specific subset of unbranched pectic (1 → 5)-α-L-arabinan (arabinanase sensitive) | Rat | Moller et al., 2008; Verhertbruggen et al., 2009b | |
| LM16 | Processed α- (1 → 5)-α-L-arabinan. Epitope might be part of galactosyl residue(s) on RG backbones (galactosidase sensitive) | Rat | Verhertbruggen et al., 2009b | |
| LM26 | (1 → 4)- β-D-galactan substituted with β-(1 → 6) galactosyl, three galactose residues required as backbone. | Rat | Torode et al., 2017 | |
| INRA-AGI-1 | RGI related linear chain of (1 → 4)-linked Gal and (1 → 5)-linked Ara | Mouse | Buffetto et al., 2015 | |
| CCRC-M11/M12/M15 | Arabinogalactan epitope on RG-I and AGP | Mouse | Pattathil et al., 2010 | |
| RG-II | RG-II | Rhamnogalacturonan II | Rabbit (pAb) | Matoh et al., 1998 |
| CCRC-R1 | Rhamnogalacturonan II (unesterified) | Phage (Fab) | Williams et al., 1996 |