Table 2.
A selection of commonly used antibodies related to hemicelluloses.
| Ab | Epitope | Source | References | |
|---|---|---|---|---|
| Mannans | BS-400-4 (BGM C6) | (1 → 4)-β-D-(galacto)mannan | Mouse | Pettolino et al., 2001 |
| LM21 | (1 → 4)-β-D-(galacto)(gluco)mannan; DP2 to DP5 | Rat | Marcus et al., 2010 | |
| LM22 | (1 → 4)-β-D-(gluco)mannan; DP2 to DP5 | Rat | Marcus et al., 2010 | |
| CCRC-M70 | Galactomannan | Mouse | Pattathil et al., 2010 | |
| CCRC-M169 | Acetylated mannan | Mouse | Pattathil et al., 2012; Zhang et al., 2014 | |
| CCRC-M170 | Acetylated glucomannan | Mouse | Pattathil et al., 2012; Zhang et al., 2014 | |
| Beta glucans | BS-400-2 (LAMP2H12H7) | (1 → 3)-β-D-glucan (callose and laminarin) | Mouse | Meikle et al., 1991 |
| BS-400-3 (BG1) | (1 → 3), (1 → 4)-β-D-glucan (MLG) | Mouse | Meikle et al., 1994 | |
| LM15 | Xyloglucan (XXXG motif), non-fucosylated (can accommodate a single Gal residue) Requires a single unsubstituted Glc on the non-reducing end | Rat | Marcus et al., 2008; Ruprecht et al., 2017 | |
| LM24 | Xyloglucan (XLLG motif) | Rat | Pedersen et al., 2012 | |
| LM25 | Xyloglucan | Rat | Pedersen et al., 2012 | |
| CCRC-M1 | α-L-fucosylated xyloglucan (also RG-I) | Mouse | Puhlmann et al., 1994; Pattathil et al., 2010 | |
| CCRC-M86/100/103 | Internal xyloglucan chain, requires non-substituted Glc residue toward non-reducing end. | Mouse | Ruprecht et al., 2017 | |
| CCRC-M93/95/96/101/104 | Xyloglucan with Gal substitution | Mouse | Dallabernardina et al., 2017 | |
| Xylans | LM10 | (1 → 4)-β-D-xylan | Rat | McCartney et al., 2005; Ruprecht et al., 2017 |
| LM11/CCRC-M147/149 | (1 → 4)-β-D-xylan/arabinoxylan, high tolerance of backbone substitutions | Rat | McCartney et al., 2005; Ruprecht et al., 2017 | |
| CCRC-M140/160/137/139/152 | (1 → 4)-β-D-xylan, low tolerance for substitutions | Rat | Ruprecht et al., 2017 | |
| CCRC-M108/109/110 | (1 → 4)-β-D-xylan substituted with Ara on the 2-position. | Mouse | McCartney et al., 2005; Ruprecht et al., 2017 | |
| LM23 | Non-acetylated xylosyl residues, pectic xylogalacturonan and xylan | Rat | Ruprecht et al., 2017 | |
| LM27 | Grass glucuronoarabinoxylan (GAX) | Rat | Cornuault et al., 2015 | |
| LM28 | (1 → 4)-β-D-xylan with GlcA substitution on 2-position, both methyl or non-methylesterified | Rat | Cornuault et al., 2015 | |
| INRA-AX1 | Backbone of xylans | Mouse | Guillon et al., 2004 | |
| INRA-UX1 | Alkali treated glucuronoxylan | Mouse | Koutaniemi et al., 2012 | |
| CCRC-M150 | (1 → 4)-β-D-xylan with GlcA substitution which is not methylesterified | Mouse | Ruprecht et al., 2017 | |
| CCRC-M144/145/146/155 | (1 → 4)-β-D-xylan with GlcA substitution which is methylesterified at 4-O-position | Mouse | Ruprecht et al., 2017 |