Figure 2.

MPP⁺ inhibits RBM3 induction by mild hypothermia in SH-SY5Y cells. With the addition of MPP⁺, cells were incubated at 32°C for 1 d. (A) The RBM3 protein level was measured by Western blot. (B) A bar graph showed the relative levels of RBM3 protein in (A). (C) The Rbm3 mRNA expression was examined by qPCR. All the data above represent the mean ± S.D. (n = 3). Multiple comparisons were analyzed by a one-way ANOVA and Newman-Keuls post hoc. ^*Indicates post hoc significant difference compared to untreated control group (32°C in the absence of MPP⁺). ^**P < 0.01 and ^***P < 0.001.