Figure 3.

Hypothermia treatment aggravates MPP⁺-induced apoptosis in SH-SY5Y cells. Cells were pretreated with hypothermia (32°C) or normothermia (37°C) for 1 d, and then treated with various concentrations of MPP⁺ for 1 d. (A) Western blot was performed to detect the level of cl. PARP. (B) Cell viability was assessed by MTT assay. (C) The apoptosis was analyzed by TUNEL to figure out the DNA fragmentation caused by MPP⁺ (3 mM). The blue shows the cell nucleus, and the red exhibits DNA fragmentation. (D) The damage was measured by Caspase-Glo®3/7 Assay to figure out the effect of hypothermia (32°C) on MPP⁺-induced apoptosis. All the data above represent the mean ± S.D. (n = 3). Multiple comparisons were analyzed by a two-way ANOVA and Bonferroni's post hoc. ^*Indicates post hoc significant difference compared to 37°C normothermia. ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001.