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. 2018 May 3;9:601. doi: 10.3389/fpls.2018.00601

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Copyright © 2018 Byun, Cui, Lee, Park, Lee, Kim and Lee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Generation and molecular characterization of Ubi:DaCBF4 transgenic rice plants. (A) Schematic representation of the binary vector construct used for DaCBF4 overexpression under the control of the maize ubiquitin promoter. RB, right border; Ubi-Pro, ubiquitin promoter; NOS-T, terminator sequence from nopaline synthase gene; 35S-Pro, CaMV 35S promoter; hph, hygromycin B phosphotransferase; T7-T, T7 terminator; LB, left border. (B) Semi-quantitative RT-PCR analysis of 6-week-old wild-type and two independent Ubi:DaCBF4 T2 transgenic plants (lines #1 and #2). (C) Genomic Southern blot analysis. Total leaf genomic DNA was isolated from wild-type and T2 Ubi:DaCBF4 transgenic rice plants. DNA was digested by BamHI and hybridized with ³²P-labeled hph probe under high-stringency conditions. (D) Overall morphology of 2-month-old wild type and T2 Ubi:DaCBF4 transgenic (independent lines #1 and #2) rice plants. Rice plants were grown under greenhouse conditions. Scale bars = 10 cm.