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. 2018 May 3;9:580. doi: 10.3389/fpls.2018.00580

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Copyright © 2018 Suzuki, Shinozuka, Hirakata, Nakata, Demura, Tsukaya and Horiguchi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Yeast two-hybrid assay using HOS15, HDA9, and PWR. Yeast cells grown on synthetic complete (SC) medium lacking leucine (Leu) and tryptophan (Trp) are shown in the left panel. Yeast cells grown on SC lacking Leu, Trp, and histidine (His), but containing 20 mM 3-amino-1,2-4-triazole (3AT) are shown in the middle panel. Combinations of vectors used are indicated in the right panel. GUS was used as a negative control. AD and DB indicate the activation domain and DNA-binding domain of GAL4, respectively.