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. 2018 May 3;9:502. doi: 10.3389/fpls.2018.00502

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Copyright © 2018 Courtial, Hamama, Helesbeux, Lecomte, Renaux, Guichard, Voisine, Yovanopoulos, Hamon, Ogé, Richomme, Briard, Boureau, Gagné, Poupard and Berruyer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A scale of automatically calculated live cells percentage. During this study, more than 550 fluorescein diacetate/propidium iodide stained carrot cell images were obtained. For each image, a percentage of live cells was calculated using our macro-based automated method. For values from 10 to 100% live cells, representative images were selected. Effective percentage of live cells is within one percent of the scale value. Red channel and contrast were then normalized for visibility. Bar: 1 mm.