Table 1.
Evaluation of raw extract toxicity of differentially aggressive fungal strains on two carrot genotypes.
| Mediuma | Strain | Concentration | Carrot genotype |
||
|---|---|---|---|---|---|
| H1 | I2 | K3 | |||
| No treatment | ++b | +++ | ++ | ||
| 25%c | + | ++ | + | ||
| C | 5% | + | +++ | ++ | |
| 1% | ++ | +++ | ++ | ||
| 0.2% | ++ | +++ | ++ | ||
| 25% | + | ++ | + | ||
| AUS001 | 5% | + | +++ | ++ | |
| 1% | ++ | +++ | ++ | ||
| 0.2% | ++ | +++ | ++ | ||
| 25% | -/+ | ++ | ++ | ||
| Carrot juice | FRA017 | 5% | + | +++ | ++ |
| 1% | ++ | +++ | ++ | ||
| 0.2% | ++ | +++ | ++ | ||
| 25% | - | + | + | ||
| FRA001 | 5% | + | +++ | ++ | |
| 1% | ++ | +++ | ++ | ||
| 0.2% | ++ | +++ | ++ | ||
| 25% | - | + | +/- | ||
| ITA002 | 5% | -/+ | ++ | ++ | |
| 1% | + | +++ | ++ | ||
| 0.2% | + | +++ | ++ | ||
| PDB | C | 25% | + | +++ | ++ |
| FRA017 | 25% | - | ++ | ++ | |
aFungal culture medium. bThe signs are as follows: (-) no embryogenesis was visible and cells were damaged, (+) early-stage embryogenic masses were visible, (++) embryos were present, (+++) embryogenesis was profuse. +/- early-stage embryogenic masses were visible, or no embryogenesis was visible depending on the repetition. The scale is illustrated in Lecomte et al. (2014), Figure 4. cResults obtained with a 25% concentration are in bold character. Carrot cell suspensions with three different genotypes were tested for embryogenesis in the presence of raw extract from A. dauci AUS001, FRA001, FRA017, and ITA002 strains grown in carrot juice medium, and in the presence of raw extract from the A. dauci FRA017 strain grown in potato dextrose broth (PDB). Embryogenesis was assessed 3 weeks after treatment. Cell suspensions either treated with uninoculated media raw extract (C) or untreated were used as an experimental control.