Table 5.
Influence of carrot genotype, fungal organic extract and aldaulactone treatments on carrot cell embryogenesis aptitude.
| Carrot genotype | Treatmenta | Evaluation of somatic embryogenesisb |
|---|---|---|
| H1 | Control | +++ |
| DMSO 0.1% | +++ | |
| Medium OE | +++ | |
| Abra 43 OE | ++ | |
| FRA001 OE | + | |
| ITA002 OE | +/- | |
| Aldaulactone 1.25 μg.mL-1 | ++ | |
| Aldaulactone 5 μg.mL-1 | + | |
| Aldaulactone 12.5 μg.mL-1 | + | |
| Aldaulactone 50 μg.mL-1 | +/- | |
| I2 | Control | ++ |
| DMSO 0.1% | ++ | |
| Medium OE | ++ | |
| Abra 43 OE | ++ | |
| FRA001 OE | + | |
| ITA002 OE | + | |
| Aldaulactone 1.25 μg.mL-1 | ++ | |
| Aldaulactone 5 μg.mL-1 | ++ | |
| Aldaulactone 12.5 μg.mL-1 | ++ | |
| Aldaulactone 50 μg.mL-1 | + | |
aThe treatments were applied as follows: control: untreated cells; DMSO: a DMSO solution at 0.1%, the DMSO concentration used to prepare OE, and aldaulactone solutions; Medium OE: organic extract of fungal culture medium; Abra 43 OE: A. brassicicola strain Abra 43 fungal culture organic extract; FRA001 OE: A. dauci strain FRA001 fungal culture organic extract; ITA002 OE: A. dauci strain ITA002 fungal culture organic extract. bThe signs are as follows: (-) no embryogenesis was visible and cells were damaged, (+/-) early-stage embryogenic masses were visible, or no embryogenesis was visible depending on the repetition, (+) early-stage embryogenic masses were visible, (++) embryos were present, (+++) embryogenesis was profuse. These notations were illustrated in Figure 4 in Lecomte et al. (2014). Carrot cell suspensions of susceptible (H1) and partially resistant (I2) genotypes were challenged with fungal organic extracts and a purified toxin candidate.