Figure 5.

Ischemic preconditioning decreases lung inflammation after small-for-size liver transplantation. Lungs were harvested 38 h after liver transplantation. ED1 and myeloperoxidase (MPO) were assessed by immunohistochemistry. Representative images of ED1 immunohistochemical staining are shown in (A). ED1-positive cells (B) and MPO-positive cells (C) after immunohistochemical staining were counted in 10 random fields per slide using a 40X objective lens. CD4 was detected by immunoblot analysis (D) and quantified by densitometry (E). Sham, lungs from sham-operated rats; FSG, lungs from full-size graft recipients; QSG, lungs from quarter-size graft recipients; QSG + IP, lungs from ischemic-preconditioned quarter-size graft recipients. Values are means ± SEM. Group sizes were 3-4 per group, a, P<0.05 vs sham operation; b, P<0.05 vs FSG; c, P<0.05 vs QSG.