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. 2018 Apr 20;10(2):95–104.

Figure 4.

Figure 4

Without using a fluorescence microscope and without UV activation, GFP was clearly seen in harvested cells after 2 days of transfection in HEK293 cells using Lipofectamine 3000 or three days of viral transduction in HUVECs using lentivirus generated from HEK293 cells utilizing Lipofectamine 3000 reagent. Two days after transfection in HEK293 cells using Lipofectamine 3000 or three days after viral transduction in HUVECs with lentivirus generated from HEK293 cells utilizing Lipofectamine 3000 reagent, the cells were harvested, and GFP was clearly in these harvested HEK293 cells (A, tube 1) and HUVECs (B, tube 1) even without using a confocal microscope or without using UV activation. On the contrary, we can not see any obvious GFP fluorescence under the same condition but using Lipofectamine 2000 (A and B, tube 2), or FuGENE 6 (A and B, tube 3) or non-transfection or non-transduction controls (A and B, tube 4).