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. 2018 Apr 20;10(2):95–104.

Figure 6.

Figure 6

High efficiency of Cx43 knockdown in HUVECs using lentivirus generated from HEK293 cells utilizing Lipofectamine 3000 transfection reagent. Immunofluorescence labeling of Cx43 in HUVECs showed significantly reduced Cx43 punctate labeling using the lentivirus generated utilizing Cx43 shRNA plasmids combined with Lipofectamine 3000 reagent (C) compared with Cx43 labeling in HUVECs using Cx43 shRNA combined with Lipofectamine 2000 transfection reagent (B) or using scrambled Cx43 shRNA plasmids with Lipofectamine 3000 reagent (A). The same result was also verified using western blot (D), there was statistically significant reduced Cx43 detection in HUVECs cells using lentivirus produced with Cx43 ShRNA and Lipofectamine 3000 transfection reagents, which showed about 90% Cx43 knocked down, compared with about 60% Cx43 knockdown using lentivirus generated utilizing Cx43 ShRNA plasmids and Lipofectamine 2000 transfection reagent (E).