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. 2018 May 3;8:135. doi: 10.3389/fcimb.2018.00135

Figure 2.

Figure 2

Effect of Dh-242 supernatant on intracellular glycerol accumulation and oxidative stress. (A) C. albicans wild type and hog1 mutant cells were resuspended in either a supernatant from Dh-242 strain grown for 48 h in YMB 3% NaCl pH 4.4 or fresh medium pre-warmed at 30°C supplemented with 1M NaCl (1.5 M final concentration). Samples were collected at 0, 1, and 3 h and intracellular glycerol was quantified. Graph shows the mean and SD from 3 independent experiments. t-test analyses were performed to show statistical significant differences ***p < 0.001, **p < 0.01, and *p < 0.05 (B) CAF2 and hog1 mutants strains were incubated in the presence of supernatant from Dh-242 strain (+) or YMB pH 4.4 (−) liquid medium at 30°C. Intracellular oxidative stress was quantified by flow cytometry using Rhodamine 123. A histogram from a representative experiment is shown. (C) Mitochondrial membrane potential was quantified using DHF after 2 h of incubation in supernatant from Dh-242 strain (+) or YMB pH 4.4 (−) liquid medium. A histogram from a representative experiment is shown. (D) A killer toxin assay was performed using supernatant from Dh-242 and Dh-246 strains grown in YMB 3% NaCl pH4.4 liquid medium for 48 h. The hog1 mutant strain carrying the empty vector (pNRUXe) or overexpressing the Catalase (pNRUX-CAT) were tested.