Figure 4.

Role of different domains of Hog1 in tolerance and response to stress. (A) C. albicans strains carrying different HOG1 gene versions were spotted on YPD plates supplemented or not with Congo red and incubated for 24 h at 30 or 37°C. (B) Tenfold cell suspensions of the indicated strains were spotted on YPD solid medium supplemented with the indicated compounds and incubated at 37°C for 24 h. (C) Exponentially growing cells were exposed to 10 mM H₂O₂ or 1M NaCl during 10 min and processed for immunoblot detection. Mkc1-P, Cek1-P, and Hog1-P indicate the phosphorylated forms of the MAPKs. Load control was detected with Anti-Myc antibody.