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Primers used in this work to generate HOG1 mutant versions.
| Mutation | Primer | Sequence |
|---|---|---|
| F321L | F321LHOGU | GAGCCTGTTTGTGAGAGTAAATTGGATTGGAGTTTTAATGACG |
| F321LHOG1 | CGTCATTAAAACTCCAATCCAATTTACTCTCACAAACAGGCTC | |
| K52R | K52R1 | CTGGTCAAAATGTTGCAGTGAGAAAAGTCATGAAACC |
| K52R2 | GGTTTCATGACTTTTCTCACTGCAACATTTTGACCAG | |
| T174A-176F | TGYAGF1 | CTTCAAGATCCACAAATGGCTGGTTTCGTGTCAACCAG |
| TGYAGF2 | CTGGTTGACACGAAACCAGCCATTTGTGGATCTTGAAC |
