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. 2018 May 3;9:860. doi: 10.3389/fimmu.2018.00860

Figure 1.

Figure 1

Survival, bacillary burden, and pathology of mice following Mtb infection. (A) Kaplan–Meier survival curve showing the death of Fth−/− mice following high dose and medium dose of Mtb infection (n = 11). (B,C) Bacillary burden in the lungs and spleens of mice at 4 and 9 weeks postinfection (n = 7). (D) Representative photograph of mouse eyes at 9 weeks postinfection (n = 5). (E) Representative H&E staining of eyes showing more vasculitis in Fth−/− mice compared to Fth+/+ mice (n = 5). (F,G) Bacillary burden in the eyes and brains of mice at 4 and 9 weeks postinfection (n = 7). (H) Representative photograph of brains from Mtb-infected mice 30 min after i.v. injection of Evans blue dye (n = 5). (I) Quantitation of Evans blue dye extracted from the brains of infected mice (n = 5). (J,K) Gross pathology of lungs and spleen of mice at 4 weeks postinfection (n = 5). (L) Representative micrographs of H&E staining of lungs from mice at 4 and 9 weeks postinfection (n = 7). Peribronchial and perivascular aggregation of histiocytes and the position of fibrous tissues in tuberculosis lesions in wild-type and knockout mice at 4 and 9 weeks postinfection. Scale bar; 500 µm. Statistical testing was performed using the unpaired Student’s t-test. Data are represented as mean ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001).