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. 2018 May 3;9:860. doi: 10.3389/fimmu.2018.00860

Figure 2.

Figure 2

Quantification of iron in mouse tissues following Mtb infection. (A–C) Iron levels in lungs, spleens, and sera of Fth+/+ and Fth−/− mice by inductively coupled plasma mass spectrometry (n = 7). (D) Hepcidin levels in the sera of mice at 4 and 9 weeks postinfection (n = 7). (E) Iron levels in urine of mice at 4 and 9 weeks postinfection (n = 7). Iron levels in urine were normalized to creatinine. (F) Iron levels in feces of mice at 4 and 9 weeks postinfection (n = 7). (G) Iron Von Giesen stain demonstrating minimal hemosiderin deposition in an Fth−/− mouse lung, representative of (n = 6). (H) Conspicuous hemosiderin deposition in an Fth+/+ mouse lung, representative of (n = 6). Inset: high-magnification iron Von Giesen stain demonstrating blue-green siderophages (arrows) and foci of hemorrhage (arrowheads) in Fth+/+ mouse. (I) Western blot showing ferroportin (FPN) in the duodenum of three mice at 4 weeks postinfection. (J) Densitometry analysis of relative FPN expression in duodenum. Expression of iron related proteins in lungs following Mtb infection. Statistical testing was performed using the unpaired Student’s t-test. Data are represented as mean ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).