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3′UTR-luciferase reporter assay. Increased luciferase activity is observed for target RNAs, but not an empty 3′ untranslated region (3′UTR) or GAPDH 3′UTR, in the presence of DAZL (blue bar) relative to an empty vector control (magenta bar). No stimulation is observed when the R115G DAZL mutant is overexpressed (green bar). Firefly luciferase signals were normalised to Renilla luciferase signals. Mean ± SEM, n = 4, **P < 0.01 (Student's t-test).
