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. 2017 Feb 18;23(3):177–186. doi: 10.1093/molehr/gax004

Figure 4.

Figure 4

(A) DAZL stimulates the translation of SYCP1, TEX11 and SMC1B. Quantitative RT-PCR of 3′UTR-luciferase expression shows that RNA stability is not affected by the presence of DAZL. Mean ± SEM, n = 4. (B) Polysome profiles. With the use of cycloheximide, polysomes are ‘frozen’ on RNA and can be seen as small bumps in the trace. Polysomes are no longer present with EDTA. (C) Percentage of 3′UTR-luciferase RNA associated with translating polysomes. In the presence of DAZL, the 3′UTRs of SYCP1 (synaptonemal complex protein-1), TEX11 (testis expressed 11) and SMC1B were significantly more ribosome bound than ribosome free, implicating DAZL in the translation of these targets. Mean ± SEM, n = 4–6. *P < 0.05, **P < 0.01 (Student's t-test).