Figure 11.

A simple model of events occurring during TE PCD. A, Related events occurring in cultured cells (Fukuda, 1997a) after hormonal induction (0 h) are displayed with time periods selected for two experiments studying the effect of probenecid and cycloheximide (Figs. 5 and 9). B, Events occurring in a single, differentiating TE are shown with an emphasis on the changes in the vacuole. A general model of TE PCD was made by Groover et al. (1997). Shaded areas indicate the distribution of fluorescein when FDA is added to the culture at stage c. CHX suppresses progression to stage b, c, and d (Fig. 9). Related events and vacuolar properties are also shown. a, Before TE differentiation; b, secondary wall formation; c, vacuole swelling, inhibition of fluorescein transport into the vacuole, and fluorescein exclusion from the vacuole; d, loss of the selective permeability of the tonoplast; e, tonoplast shrinkage and fragmentation; f, a dead TE.