Figure 3.

Exclusion of fluorescein from the TE vacuole. Cells cultured for 0 (A), 50 (B), or 58 h (C) were stained with FDA for the indicated times at 26°C. The percentages of TEs and non-TE cells that do not contain fluorescein in their vacuole at all were calculated. Cells from the same culture but from another batch containing FDA from the start were sampled at indicated times and examined for the presence of fluorescein in vacuoles (D). The percentages of total TEs and the ratios of dead to living TEs in cultures in the presence or absence of FDA are shown in E. Asterisks (*) indicate that the parameter could not be defined at this time because TE formation had not yet occurred. Error bars represent sd.