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. 1999 Nov;121(3):763–774. doi: 10.1104/pp.121.3.763

Figure 6.

Figure 6

Effect of pH and probenecid concentration on cell viability. A, Dependence of the probenecid effect on medium pH. Probenecid at 100 μm was added at 53 h to culture media, the pH of which was adjusted to 4.5 or 5.5 with the appropriate amounts of 1 n KOH just after probenecid addition. The percentages of TE and non-TE cells were examined separately at 1 and 3 h after the addition of probenecid. T(53) = 23.62 ± 4.69, L(53) = 18.34 ± 3.64. B, Dependence of the probenecid effect on the probenecid concentration under the same pH (4.7) medium. The medium of zinnia cell culture was buffered with 20 mm MES (pH 4.7) at 53 h after induction and supplied with 100 to 1 μm probenecid. The percentages of TE and non-TE cells were calculated separately 1 and 3 h after the treatment with probenecid. T(53) = 23.62 ± 4.69, L(53) = 18.34 ± 3.64. Error bars represent sd.