Figure 2.

EC at 30 μmol/L protects against ROS in INS-1 cells. (A) INS-1 cells incubated for 24 h with or without EC or NAC were exposed to low or high glucose medium for 3 h in the continuous presence or absence of EC or NAC. The antioxidant status of the cells was then determined by measuring intracellular ROS accumulation after loading the cells with 5 μmol/L of the ROS dye DCFDA for 30 min. (B) INS-1 cells incubated for 24 h with or without EC or NAC were loaded with 5 μmol/L of the fluorescent ROS dye DCFDA for 30 min. The cells were then exposed to 50 μmol/L H₂O₂ to increase intracellular ROS for 2 min. The results were expressed in arbitrary units of fluorescence emission at 535 nm. Six to eight independent experiments were run in which each condition was tested in quadruplicate. The data were analyzed using one-way ANOVA with Bonferroni's multiple comparisons. ^*P<0.05.