Figure 3.

EC (0.3 μmol/L) enhances insulin secretion from INS-1 cells treated with SFA. INS-1 cells were incubated for 24 h with or without EC in the continuous presence or absence of SFA. Then, the cells were exposed to low or high glucose medium for 90 min in the continuous presence or absence of EC after a quiescent period of 90 min. (A) Insulin secreted into the medium and remaining in the cells was measured by RIA to calculate the % release at each glucose concentration. (B) Total insulin synthesized was calculated as the sum of insulin secreted and remaining in the cells. Five independent experiments were run in which each condition was tested in triplicate. The data were analyzed using two-way ANOVA with Bonferroni's multiple comparisons. ^*P<0.05.