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Effect of enzyme concentration on metabolism of [14C]GA20
| Fraction | Relative Enzyme Concentration
|
||
|---|---|---|---|
| 100× | 10× | 1× | |
| GA20 | 6.4 | 6.9 | 24.5 |
| GA29 | 66.6 | 79.5 | 74.0 |
| GA29-catabolite | 26.4 | 13.6 | 1.4 |
Lysate of bacteria expressing GA 2-oxidase was diluted with inactive control lysate and incubated overnight at 20°C with substrate and cofactors. Products were separated by HPLC. Results are expressed as percentages of total counts in substrate and product fractions.
