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. 2018 Mar 22;39(5):845–857. doi: 10.1038/aps.2017.182

Table 2. Comparison of various methods used for preservation of organs.

Methods Temperature Organ Merits Challenge Clinical application Reference
Cold static storage 0–8 °C Kidney, liver, lung, heart Low cost, simple and easy to operate Metabolite accumulation, not for organ function assessment Kidney, liver, lung, heart 32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50
Hypothermic machine perfusion 0–8 °C Kidney, liver, lung, heart Provides oxygen and metabolic substrates Perfusion time is limited, not for organ assessment Kidney, liver 28,29,54,55,56,57,58
Normothermic machine perfusion 35–38 °C Kidney, liver, lung, heart Provides oxygen and essential substrates; maintains metabolic activity and viability; good for organ assessment and repair Perfusion time is limited for organ regeneration Kidney, liver, lung, heart 8,10,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78
Subnormothermic machine perfusion 20–34 °C Kidney, liver Newly proposed technique To be determined 85,86,87,88,89
Controlled oxygenated rewarming 8–20 °C Kidney, Liver Slowly, gradually rise the perfusate temperature to mitigate ischemia reperfusion injury To be determined Liver 90,91,92