Table 1.

The effects of the essential oil components on the acetylcholinesterase activity in insects.

No.	Essential Oils Components	AChE Source	IC50 (mM)	Ki (mM)	Reference
1	Anisaldehyde	BxACE-1 from Bursaphelenchus xylophilus	4.95		[46]
		BxACE-2 from Bursaphelenchus xylophilus	8.53		[46]
		BxACE-3 from Bursaphelenchus xylophilus	>50		[46]
2	Camphene	Blatella germanica	N.A.		[47]
3	Camphor	Blatella germanica	N.A.		[47]
4	3-Carene	BxACE-1 from Bursaphelenchus xylophilus	0.37		[46]
		BxACE-2 from Bursaphelenchus xylophilus	8.18		[46]
		BxACE-3 from Bursaphelenchus xylophilus	>50		[46]
5	Carvacrol	Musca domestica	0.0012		[48]
		Dermacentor variabilis	0.0018		[48]
		Periplaneta americana	0.0004		[48]
		Aedes aegypti	0.0012		[48]
		Drosophila suzukii	N.A.		[49]
		Sitophilus oryzae		0.05	[50]
6	Caryophyllene (humulene)	Blatella germanica	N.A.		[47]
7	1,8-Cineole	Pediculus humanus capitis	77		[51]
		Sitophilus oryzae		0.084	[50]
8	Coniferyl alcohol	BxACE-1 from Bursaphelenchus xylophilus	1.06		[46]
		BxACE-2 from Bursaphelenchus xylophilus	1.41		[46]
		BxACE-3 from Bursaphelenchus xylophilus	1.13		[46]
9	Cymene	Sitophilus oryzae		0.05	[50]
		Drosophila suzukii	N.A.		[49]
10	Estragole (Allylanisole)	Blatella germanica	N.A.		[47]
11	Eugenol	Sitophilus oryzae		0.096	[50]
12	Isoeugenol	Sitophilus oryzae		0.11	[50]
13	Isosafrole	Sitophilus oryzae		0.71	[50]
14	Limonene	Sitophilus oryzae		0.73	[50]
		Reticulitermes speratus Kolbe	0.95		[41]
15	Linalool	Sitophilus oryzae	N.A.		[50]
16	Methyleugenol	Sitophilus oryzae		0.051	[50]
17	Menthol	Sitophilus oryzae		0.048	[50]
		Drosophila suzukii	N.A.		[49]
18	Menthone	Sitophilus oryzae		0.39	[50]
		Drosophila suzukii	N.A.		[49]
19	Nerolidol	BxACE-1 from Bursaphelenchus xylophilus	9.98		[46]
		BxACE-2 from Bursaphelenchus xylophilus	15.28		[46]
		BxACE-3 from Bursaphelenchus xylophilus	19.06		[46]
20	Nootkatone	Musca domestica	>30		[48]
		Dermacentor variabilis	>30		[48]
		Periplaneta americana	>30		[48]
		Aedes aegypti	>30		[48]
21	Ocimene	Japanese termite	0.96		[52]
		Blatella germanica	N.A.		[47]
22	Perilla aldehyde	Drosophila suzukii	3.06		[49]
23	Phellandrene	Reticulitermes speratus Kolbe	4.92		[41]
		Blatella germanica	2.2		[47]
24	α-Pinene	Sitophilus oryzae		0.44	[50]
		BxACE-1 from Bursaphelenchus xylophilus	0.24		[46]
		BxACE-2 from Bursaphelenchus xylophilus	0.64		[46]
		BxACE-3 from Bursaphelenchus xylophilus	0.68		[46]
		Reticulitermes speratus Kolbe	3		[41]
25	β-Pinene	BxACE-1 from Bursaphelenchus xylophilus	3.39		[46]
		BxACE-2 from Bursaphelenchus xylophilus	18.03		[46]
		BxACE-3 from Bursaphelenchus xylophilus	>50		[46]
		Reticulitermes speratus Kolbe	3.08		[41]
		Sitophilus oryzae		0.0028	[50]
26	α-Terpinene	Sitophilus oryzae		0.14	[50]
27	α-Terpineol	Sitophilus oryzae		3.94	[50]
28	β-Thujone	Blatella germanica	N.A.		[47]
29	Thymol	Sitophilus oryzae		0.57	[50]
		Drosophila suzukii	4.26		[49]

N.A.—the compound is not active or the inhibition is lower than 50%; IC₅₀—Concentration of component that cause 50% inhibition of enzyme; Ki—inhibitory constant. BxACE-1, BxACE-2 and BxACE-3 are three different acetylcholinesterases found in Bursaphelenchus xylophilus. Values in mg/mL were recalculated by the authors of this paper.