Table 3.
| Genes | Proteins | MiRNAs * |
|---|---|---|
| Putative target transcripts of miRNAs upregulated in CPT2-deficient fibroblasts: | ||
| SIRT1 | SIRT1 | 2 × 449b/539/9/651/ |
| STK11 | LKB1 | 483/ |
| PRKAA1 | AMPK subunit | 301/449b/539/371/9/651/ |
| PRKAA2 | AMPK subunit | 483/301/2 × 449b/206/4 × 371/3 × 10b/3 × 651/ |
| PRKAB1 | AMPK subunit | 483/301/9/ |
| PPARGC1A | PGC-1 α | 2 × 301/539/196a-2/ |
| ALDH7A1 | PDE | 2 × 483/449b/2 × 371 2 × 10b/651/ |
| ESR1 | ER | 483/3 × 301/2 × 206/ 371/2 × 9/196a-2/ |
| ESRRA | ERR α | 449b/ |
| NRF1 | NRF1 | 483/449b/2 × 539/3 × 371/9/ |
| NFE2L2 | NRF2 | 651/ |
| TFAM | TFAM | 483/206/539/2 × 371/10b/9/651/ |
| Putative target transcripts of miRNAs downregulated in CPT2-deficient fibroblasts: | ||
| SIRT1 | SIRT1 | 211/141/136/181a2,c/496/126-5p/ |
| STK11 | LKB1 | - |
| PRKAA1 | AMPK subunit | 496/126-5p/144/ |
| PRKAA2 | AMPK subunit | let-7a3,d,g/2 × 141/203/3 × 181a2,c/4 × 126-5p/144/9/ |
| PRKAB1 | AMPK subunit | 2 × 141/2 × 203/181a2,c/ |
| PPARGC1A | PGC-1 α | let-7a3,d,g/211/141/136/203/496/3 × 126-5p/144/ |
| ALDH7A1 | PDE | 141/2 × 136/203/ |
| ESR1 | ER | 211/136/203/181a2,c/496/ |
| ESRRA | ERR α | - |
| NRF1 | NRF1 | 2 × 211/181a2,c/ |
| NFE2L2 | NRF2 | 181a2,c/496/ |
| TFAM | TFAM | 211/4 × 141/2 × 136/2 × 203/181a2,c/496/4 × 126-5p/4 × 144/ |
| Putative target transcripts of miRNAs upregulated after RSV treatment of CPT2-deficient fibroblasts: | ||
| SIRT1 | SIRT1 | 199a1-5p/ |
| STK11 | LKB1 | 199a1-5p/ |
| PRKAA1 | AMPK subunit | - |
| PRKAA2 | AMPK subunit | 219/2 × 299/193a/199a1-5p/2 × 337/ |
| PRKAB1 | AMPK subunit | 193a/ |
| PPARGC1A | PGC-1 α | 219/193a/2 × 199a1-5p/ |
| ALDH7A1 | PDE | 199a1-5p/2 × 337/ |
| ESR1 | ER | 219/299/2 × 193a/337/ |
| ESRRA | ERR α | - |
| NRF1 | NRF1 | 199a1-5p/ |
| NFE2L2 | NRF2 | 337/ |
| TFAM | TFAM | 299-5p/2 × 193a/199a1-5p/2 × 337/ |
| Putative target transcripts of miRNAs downregulated after RSV treatment of CPT2-deficient fibroblasts: | ||
| SIRT1 | SIRT1 | 181d/23a/ |
| STK11 | LKB1 | 20b/17-5p/ |
| PRKAA1 | AMPK subunit | 2 × 101-1/2/16-1/21/26a/ |
| PRKAA2 | AMPK subunit | 3 × 181d/21/20b/let-7c,d/17-5/2 × 146a/376b/26a/23a/ |
| PRKAB1 | AMPK subunit | 181d/146a/ |
| PPARGC1A | PGC-1α | 101-1/2/let-7d/376b/26a/2 × 23a/ |
| ALDH7A1 | PDE | 16-1/2 × 20b/2 × 17-5p/146a/ |
| ESR1 | ER | 181d/21/3 × 20b/3 × 17-5p/146a/2 × 26a/2 × 103-1/23a/ |
| ESRRA | ERRα | 16-1/103-1/423/ |
| NRF1 | NRF1 | 181d/2 × 21/ |
| NFE2L2 | NRF2 | 181d/103-1/ |
| TFAM | TFAM | 181d/3 × 20b/3 × 17-5p/2 × 376b/26a/23a/ |
* MiRNAs are given in the same order as in Table 1 and Table 2. The sign “x“ indicates the number of putative target sequence for a given miRNA. Target transcripts were identified using the Targetscan software (www.targetscan.com). Numbers in front of miRNAs indicate that more than one consensus target site for this miRNA is present in the 3′-untranslated region of the transcript. For instance, SIRT1 3-untranslated region contains two consensus target sequences for miR-449b.