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. 2018 May 9;38(19):4505–4520. doi: 10.1523/JNEUROSCI.3528-17.2018

Figure 3.

Figure 3.

Repeated NPY treatment diminishes resting Ih in BLA PNs. A, Top, Superimposed current responses to families of hyperpolarizing voltage clamp steps (bottom) from Vh = −55 mV from a representative Veh-treated (black traces) and NPY-treated (gray traces) neuron 2W after the first injection. Inset, Illustration of Ih measurement (response to a step to −135 mV). B, I–V relationship of Ih from neurons (numbers as indicated) from 2W Veh-treated (white circles) and NPY-treated (gray squares) rats. Data from 25 and 16 rats, respectively, are shown; cell numbers are as indicated (2-way RM-ANOVA: treatment: F(1,78) = 0.645, p = 0.42; voltage: F(7,546) = 334.9, p < 0.0001; interaction: F(7,546) = 0.292, p = 0.96. C, Top, Superimposed current traces to voltage steps (bottom) as in A for representative neurons from animals 4W after treatment with Veh (black traces) or NPY (gray traces). D, I–V relationship of Ih from 4W Veh- (white circles) and NPY-treated animals (gray squares) (Veh n = 55 cells/34 rats, NPY n = 50/20; 2-way RM-ANOVA: treatment: F(1,104) = 17.67, p < 0.0001; voltage: F(7,728) = 285.9, p < 0.0001; interaction: F(7,728) = 12.78, p < 0.0001. Bonferroni's multiple-comparisons test; C,D). E, F, Fractional activation of Ih versus step potential for neurons from 2W (E) and 4W (F) Veh- and NPY-treated animals. Boltzmann fits and corresponding midactivation voltages (V1/2) indicated no significant differences in fractional activation between neurons from Veh- and NPY-treated animals (2W: Veh: −105.4 ± 1.1 mV, n = 48 neurons from 25 rats; NPY: −102.0 ± 1.4 mV, n = 24 neurons from 16 rats; unpaired t test: t(70) = 1.81, p = 0.075; 4W: Veh: −103.3 ± 1.3 mV, n = 49 neurons from 34 rats; NPY: −105.9 ± 1.6 mV, n = 38 neurons from 20 rats; unpaired t test: t(85) = 1.25, p = 0.22). In B and D–F, numbers of individual neurons/rats tested are as indicated in legends. Data in B–F are represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, Bonferroni's multiple-comparisons test or t test where appropriate.