Figure 6.

Effect of GA₁ application on light regulation of GA 20-ox and GA 3β-hy transcript accumulation. A, Effect of 30 min of exposure to dim-green safelight (G) on transcript accumulation in apical buds. B, Exogenous application of GA₁ on apical buds of 6-d-old etiolated seedlings 30 min prior transfer to light. Light treatment and tissue harvestings were identical to Figure 3A. C, Two micrograms of GA₁ was applied to the apical buds of 6-d-old etiolated seedlings 30 min prior to the red light pulse. Light treatments were identical to Figure 5. RNA-blot analysis was performed as described in Figure 2. D, Darkness; L, light; R, red light.