Fig. 1.

Expression of SPIN1 in breast cancer cells and tissues. a The Adriamycin resistant breast cancer MCF-7/ADM cells displayed greater resistance to Adriamycin than MCF-7 cells (IC50: 29.17 vs 1.179). b SPIN1 was highly expressed in Adriamycin resistant MCF-7/ADM cells, and metastatic MDA-MB-231 and MDA-MB-468 cells, compared with MCF-7 cells (^*P < 0.05). c-e Drug-resistant (d) tissues displayed higher SPIN1 protein levels than drug-sensitive (c) tissues (e, P = 0.0020, scale bar = 50 μm). f Receiver operating characteristic (ROC) curve analysis showed that SPIN1 could significantly distinguish drug-resistant tissues from drug-sensitive tissues (area under the curve (AUC) = 0.7025, P = 0.002096). g Data from GEO datasets GSE22513 showed that SPIN1 was highly expressed in non-pCR patients compared to pCR (pathologic complete response) patients. h Data from GSE18728 showed that SPIN1 expression was higher in non-responders than responders. i Analysis of the GEO dataset METABRIC showed that high expression of SPIN1 predicted poorer survival of breast cancer patients