Fig. 2.

Effect of the H₂S donor NaSH on CBS, CSE and MPST mRNA in human ASM cells from non-smokers, smokers and COPD patients. ASM cells were incubated with FCS (2.5%) for 1 h and NaSH (100 μM) was added for another 24 h. Change in CBS (a), CSE (b), and MPST (c) mRNA expression was subsequently measured by TaqMan RT-PCR. Bars represent means (± SEM) of nine ASM donors. ^*/+/#P < 0.05; ^**/++/##P < 0.01; ^***/+++/###P < 0.001