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. 2018 May 9;18:152. doi: 10.1186/s12906-018-2204-y

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — The effect of AC on the mRNA expression of skp2 and RhoA and the protein expression of PARP and skp2. a-d Total mRNA was extracted from the two breast cancer cells after treat without or with indicated drugs for 24 h. The coding regions of human Skp2 and RhoA were used as probes for real time polymerase chain reaction analysis. e, f Total cell extracts of the two breast cancer cells were harvested from cells treated with DMSO or indicated concentrations of AC for 24 h. The protein was immunoblotted with polyclonal antibodies specific for PARP or Skp2. β-actin was used as an internal loading control. (Error bars = mean ± S.E.M. Asterisks (*) mark samples significantly different from DMSO group with p < 0.05)