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. 2018 May 3;9:598. doi: 10.3389/fpls.2018.00598

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Copyright © 2018 Shivalingamurthy, Anangi, Kalaipandian, Glassop, King and Rae.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic relationships of plant INVINH proteins. The phylogenetic tree was constructed by neighbor joining using aligned INVINH sequences analyzed by MEGA7.0. Asterisk (^*) indicates invertase inhibitors characterized experimentally. ShINH1 & ShINH2 are shown in boxes. Dicot (I and III) and monocot (II and IV) INVINH proteins alignment are shown in lines. INVINH-like proteins from Solanum tuberosum (StINVINH2A, GU321341; StINVINH2α, KJ788176; StINVINH2αA2, FJ810206; StINVINH2αA1, FJ810205; StINVINH2αB, FJ810207; StINVINH2b1, ADZ54776; StINVINH2b2, GU980595; StINVINH2B, GU321342; StINVINH2αC, FJ810208; StINVINH2αD, FJ810209; StINVINH PMEI LIKE,); Solanum lycopersicum, (KC007445); Nicotiana tobacum (NtVINVINH, AY145781; NtINVINH, AY594179; NtINVINH2, Y12805; NtVIF, AAN60076; NtINVINH1, Y12806); S. spontaneum (SspoINH, KP844455); Aegilops tautschi (AegtauINH, XM_020320985); S. robustum (SrINH, KP055631); S. sinense (SsineINH, KP997206); S. officinarum (SoINH, KP997207); S. barberi (SbarINH, KU057162); Saccharum hybrid (ShINH2, MG457817); Zea mays (ZmINVINH3, CAC69343); A. tautschi (AegtauINH1, XM_020311699); Z. mays (ZmINVINH2, CAC69336); Arabidopsis thaliana (AtINH, Y12807); Sorghum bicolor (SbINHCWI/VAI, XM_002446958.2); Setaria indica (SiPMEI, XM_004978185.1); Z. mays (ZmINVINH1; ZmINHCWI/VAI, XM_008670754.2) and Saccharum hybrid (ShINH1, MG457818; SoINVINH1, KF575171;). A total of 1,000 bootstrapping runs were performed and % reliability is labeled next to each branch. ShINH1 and ShINH2 clustered within the Saccharum spp. and were closely related to monocot INVINH proteins.